Calculate the volume of compound y needed to make a 350 pg/ml treatment solution in 2 ml of 1x pbs using a stock solution containing 50 ml of compound y at 50 pg/ml. 14 l not possible to make as described 140 pl 1.4 mi

14 mL

Explanation:

To prepare a solution by a concentrated solution, we must use the equation:

C1xV1 = C2xV2, where C is the concentration, V is the volume, 1 is the initial solution and 2 the final solution.

The final solution must have 2 mL and a concentration of 350 pg/mL, and the initial solution has a concentration of 50 pg/mL.

Then:

50xV1 = 350x2

50xV1 = 700

V1 = 700/50

V1 = 14 mL

1. for the first question, we must find the mass of the anhydrous salt, mgso₄. the molar mass for mgso₄·7h₂o is 246.47 g/mol, while that of mgso₄ is 120.37 g/mol.7.834 g mgso₄·7h₂o * 1 mol mgso₄·7h₂o/246.47 g * 1 mol mgso₄/1 mol mgso₄·7h₂o * 20.37 g mgso₄/mol = 0.647 g mgso₄

2. mass of water = mass of sample - mass of anhydrous salemass of water = 7.834 - 0.647 = 7.19 gpercent water in hydrate = 7.19/7.834 * 100 = 91.74%