During the phosphatase experiment you will use a 1% w/v of phenolphthalein di-phosphate (ppp). how much ppp do you need to make 500ml of the solution if you only have 25g and you know that the mw of ppp is 478 g/mole?

To make 500 mL of a solution of PPP 1% w/v you need 5g

Explanation:

A percent w/v solution is calculated with the following formula using the gram as the base measure of weight (w):

% w/v = g of solute/ mL of solution × 100

A 1% w/v means that you have 1 g of PPP per 100 mL of solution

Thus, if you need to make 500 mL of solution you will need 5 g of PPP.

I hope it helps!