Given information:
resin used in this experiment is a strong anion exchanger.
two proteins being separated are: cytochrome c (pi range 10.0-10.5) and ferritin (pi is 5.3)
choose one buffer from the following that would be best for this experiment. buffer must be ph 7.0 because both proteins are stable at ph 7.0
1. diethylamine - buffering range 9.5--11.5
2. lactic acid - buffering range 3.6--4.3
3. phosphate - buffering range 6.7--7.6
4. tricine - buffering range 7.8--8.9
5. tris - buffering range 7.0 - 8.0
consider the type of ion exchanger, the pi of the proteins, the ph of the buffer and the buffering range and type of buffer provided when making your choice. explain why you chose one over the other.