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Business, 15.02.2020 03:40 tednequamoore4307

A nonapeptide was determined to have the following amino acid composition: (Lys)2, (Gly) 2, (Phe) 2, His, Leu, Met. The native peptide was incubated with 1- fluoro-2,4-dinitrobenzene (FDNB) and then hydrolyzed; 2,4- dinitrophenylhistidine was identified by HPLC. When the native peptide was exposed to cyanogen bromide (CNBr), an octapeptide and free glycine were recovered. Incubation of the native peptide with trypsin gave a pentapeptide, a tripeptide, and free Lys. 2,4-Dinitrophenyl-histidine was recovered from the pentapeptide, and 2,4- was recovered from the tripeptide. Digestion with the enzyme pepsin produced a dipeptide, a tripeptide, and a tetrapeptide. The tetrapeptide was composed of (Lys) 2, Phe, and Gly. What is the native sequence of the peptide?

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A nonapeptide was determined to have the following amino acid composition: (Lys)2, (Gly) 2, (Phe) 2,...
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