Experimental evidence indicates that glyceraldehyde-3-phosphate dehydrogenase contains a critical residue in its active site, as shown by its inactivation with iodoacetate.

Experimental evidence shows glyceraldehyde-3-phosphate dehydrogenase has a critical Cysteine residue in it's active site as being shown with the inactivation with iodoacetate. Cysteine is an amino acid with sulfur in humans. It is vital in protein synthesis, metabolic function and detoxification. It is also important for collagen production along with skin texture.