You decide to clone a gene using a plasmid vector featuring ampicillin resistance and lacz. however, when you try to grow the e. coli onto a plate containing x-gal and ampicillin, you see no colonies. explain what most likely went wrong during the cloning process.

Which best explains why viruses do not have special structures or enzymes that allow them to make their own food? viruses can use energy in living cells that they infect. viruses can replicate inside a host that they infect. viruses can cause contagious illnesses in host cells. viruses integrate their rna or dna into infected cells.

The graph shows the amount of global warming happening in different ecosystems. which populations of organisms will likely decline the most as a result of climate change, and why? a.coral reefs because of the loss of the symbiotic algae living within their bodies b.penguin populations because the fish they eat will move into warmer waters c.mountain-dwelling birds because rising sea levels will relocate their food sources d.large cats in the rainforest because of decreased habitat loss for their prey

Which member of the following food chain will be least affected by ddt, a pesticide water pollutant, if bio-magnification is occurring? algae> zooplankton> crayfish> leopard frog> large mouth bass