What is the effect of boiling the chloroplast suspension on photosynthetic activity and why?

Boiling chloroplasts prevents the DPIP from being reduced because the enzymes for photosynthesis are no longer present in the chloroplasts. DPIP is reduced from blue to colorless when light strikes the chloroplasts and the electrons are boosted to a higher energy level. Since photosynthesis cannot be performed by the denatured chloroplasts, the DPIP cannot be reduced.

Other organisms can change their behavior to take the place of the plants in the ecosystem.