While testing amylase activity, john uses a buffer of ph 6 and substrate starch. he incubates them at 104ºf (40ºc). all the tubes show glucose formation, except one, which had no starch solution. why did this happen?

the principles and empirical processes of discovery and demonstration considered characteristic of or necessary for scientific investigation, generally involving the observation of phenomena, the formulation of a hypothesis concerning the phenomena, experimentation to test the hypothesis, and development of a conclusion that confirms, rejects, or modifies the hypothesis.

. ph changes the ability of them he enzyme to dissolve in water.