Arrange the following statements to create the proper order of events in the humoral immune response: 1. the t cell interacts with antigen presented in an mhc-ii molecule. 2. the b cell divides to produce memory b cells and effector b cells. 3. antibodies specific for the antigen are produce in large numbers. 4. the t cell secretes interleukin-2. 5. the b cell presents antigen fragments specific for its surface antibodies using mhc-ii molecules on the cell surface. 1, 3, 2, 5, 4 5, 1, 4, 2, 3 3, 4, 2, 5, 1 4, 2, 5, 1, 3 2, 4, 3, 1, 5

you are certainly talking about this picture:

a: is the label of the white square, it is showing the  replication fork, formed by helicase (d) ==> so it is the site of dna separation and replication 

b: is a new dna brand that is following directly the replication fork, so it is continuous ==> built continuously from 5' to 3

c: dna polymerase cannot replicate dna on its own, it needs the of their proteins, and one of them plays the role of a clamp which fixes  the dna with the dna template and increases  the processivity of the enzyme. ==>   stops polymerase from falling off the  template.

d: is the enzyme responsible for forming the replication fork (helicase) ==> unwinds and separates dna into two strands

e: is the delayed strand, it is built  in the opposite sense of the helicase, so dna will be built in fragments ==> built  fragments from 5' to 3 joins

and finally f: is the enzyme that builds  the new dna strand (dna polymerase) ==>   adds nucleotides to make a new dna helix.

credit: gradman555p98x9p

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protocol for imps extraction focusing on membrane protein extraction, solubilisation, and purification.