In a living organism, at which stage does segregation of gene pairs takes place

Segregation of gene pairs occurs during meiosis.

Explanation:

Almost all cells that make up an individual have paired chromosomes, called homologous chromosomes. The gametes of this individual, however, have only one chromosome from each pair. This reduction in the number of chromosomes occurs because the cells that give rise to gametes undergo meiosis, a type of cell division in which, in one step, the separation (segregation) of homologous chromosomes occurs, where segregation of gene pairs occurs.

Since genes are located on chromosomes, the separation of homologues is also the separation of genes, those genes located on the same locus as homologous chromosomes and related to determining the same trait.

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