Suppose you wish to test a species of bacteria to see if it grow under anaerobic conditions. you know that this species grows best at night temperatures and acidic ph. it does not grow well at low temperatures and alkaline ph. describe your experimental setup.

First, the source or type of specimen should be considered in selecting the medium. Anaerobic microorganisms are handled best in an oxygen-free transport tubes, rubber stopper vials, or anaerobic pouch. The information gathered above about the species should be noted because they are also helpful in selecting the medium. The appropriate set up should met the requirements available to grow the species. Microorganisms that grows best at cold temperature are called psychrophiles. This experiment should be done at night and the use of lactic acid and nutrient rich media is necessary. DeMann Rogosa and Sharpe's medium are used specifically in growing lactic acid bacterira.

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