1. What are two major challenges to the traditional five kingdom system?

You have been asked to lead a demonstration for the undergraduate microbiology lab course about the uses of negative staining when studying bacteria. a "negative" stain does not stain the bacterial cell itself but stains the space between cells. under magnification, the acidic (negativelycharged) nature of the stain will be repelled by the negatively charged bacterial cell wall and willleave the cell colorless in a stained background. negative stains are used primarily to reveal the presence of negatively charged bacterial capsules; therefore, they are also called capsule stains. encapsulated cells appear to have a halo surrounding them. the negative stain procedure does not require heat fixation, which limits any chances of alteration in bacterial cell shape and size. the bacterial suspension is added to a drop of stain, such as nigrosin or eosin, and drawn across the glass slide using a coverslip.nigrosin staining-not safranin staining-of klebsiella pneumoniae will allow for the visualization of the cell shape and the determination of the presence of a capsule.true/false

The endoplasmic reticulum (er) is an organelle in some cells. in these cells, it is continuous with the nuclear membrane and forms a large part of the cell\'s total membrane. sort the phrases below as describing rough er, smooth er, both, or neither. note: if you answer any part of this question incorrectly, a single red x will appear indicating that one or more of the phrases are sorted incorrectly.1) found in prokaryites and uekaryotys2) involved in protein synthesis3) involve in lipid synthesis4) contains molecular chaperones in lumen5) bound with ribosomes6) involved in toxin or drug metabolism7) large systemot internnected tubules,vesicles, and sacs8) "packages" enzymes in vesicles for secretion.

Did you know that a blue wales tongue weighs more than an elephant?