Biology, 17.12.2021 03:40 COOLIOMARIS

g The variation in the numbers of phage resistant mutants (among different E. coli cultures) seen in the Luria-Delbruck fluctuation test is a reflection of: Group of answer choices Differing levels of mutagenesis in the different cultures The differing media in which the bacterial cultures were grown The concentration of phages in the phage suspension added to the cultures The growth phase of the bacterial cultures, before being challenged with phage When the mutation(s) conferring resistance arose (i. e. early or late), during growth of the different cultures

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you are certainly talking about this picture:

a: is the label of the white square, it is showing the replication fork, formed by helicase (d) ==> so it is the site of dna separation and replication

b: is a new dna brand that is following directly the replication fork, so it is continuous ==> built continuously from 5' to 3

c: dna polymerase cannot replicate dna on its own, it needs the of their proteins, and one of them plays the role of a clamp which fixes the dna with the dna template and increases the processivity of the enzyme. ==> stops polymerase from falling off the template.

d: is the enzyme responsible for forming the replication fork (helicase) ==> unwinds and separates dna into two strands

e: is the delayed strand, it is built in the opposite sense of the helicase, so dna will be built in fragments ==> built fragments from 5' to 3 joins

and finally f: is the enzyme that builds the new dna strand (dna polymerase) ==> adds nucleotides to make a new dna helix.

credit: gradman555p98x9p

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