You are the resident biochemical scientist at a pharmaceutical company and you are part of a research group working on drug development targeting pancreatic cancer. You come across the attached review (Murthy et al. 2018) on the role of PI3K signaling in PDAC, the most aggressive form of pancreatic cancer. Your company has access to a pancreatic cancer cell line and a healthy pancreatic cell line that you can grow and manipulate. Your company also has access to a 2 million compound library to screen for small molecule drugs against any protein of interest. Your job is to read the above-mentioned review carefully and propose two sets of experiments (as explained in 2A.-2C. below) based on the assays available at your company. Available protein-based assays: a. Ability to delete a protein of choice from these cell lines. b. Ability to overexpress or introduce a protein of choice into these cell lines. c. Ability to measure cell growth and death. d. Ability to measure ligand-protein binding on-rate (kon) and off-rate (koff) constants. e. Ability to measure protein function for any protein of interest (a tall order in practice!). Target identification experimental design: 2A. Use the review to hypothesize about two of the proteins that can serve as promising therapeutic targets for PDAC. Then design experiments that will test your hypothesis. Drug discovery experimental design: 2B. We will now assume that you have identified those two proteins as potential targets to inhibit for PDAC. Now create an experimental design using the above-mentioned protein-based assays to find small molecule drugs that will serve as inhibitors for those proteins. 2C. Will these drugs work in cancer patients who have mutations in those proteins? If not, what is a potential work-around? What are the potential benefits or drawbacks of using these two drug candidates together in a drug combination?

The process by which natural forces move weathered rock and soil brim one place to another is called

Question 1(multiple choice worth 3 points) (06.04 lc) which of the following is true about repetition in an experiment? it involves setting up of a hypothesis. it involves multiple trials. it involves data analysis. it involves peer review. question 2(multiple choice worth 3 points) (06.03 mc) the trait for flower color in a plant has red and white alleles. the red color is the dominant trait. what is the phenotypic ratio for a cross between plants with red (rr) flowers and white (rr) flowers? 4 red : 0 white 3 red : 1 white 2 red : 2 white 0 red : 4 white question 3(multiple choice worth 3 points) (06.01 mc) the following table describes some biotechnological processes. biotechnological processes process description 1 producing offspring that have identical dna as one parent 2 crossing a high-yielding fruit variety with a high-vitamin variety which biotechnological processes are described in the table? both processes describe cloning. both processes describe genetic engineering. process 1 describes cloning and process 2 describes artificial selection. process 1 describes artificial selection and process 2 describes cloning. question 4(multiple choice worth 3 points) (05.04 mc) which of the following adaptations best polar bears walk on snow? furry feet slippery feet small feet white-colored feet question 5(multiple choice worth 3 points) (06.01 hc) a team of scientists altered the dna of e. coli bacteria. the bacteria thus developed had the ability to generate insulin exactly identical to the human insulin molecule. what could be the likely impact of this biotechnology on the individual and the society? the medical costs for insulin-dependent patients would rise. large-scale insulin could be produced in relatively lesser time. the price of insulin available from sources other than bacteria may fall. the patients who take insulin may become infected with e. coli bacteria. question 6(multiple choice worth 3 points) (06.03 lc) which of the following is used to represent a recessive trait in a punnett square? an uppercase letter a lowercase letter a shaded square a shaded circle question 7(multiple choice worth 3 points) (06.03 mc) cystic fibrosis is a recessive gene disorder. the pedigree chart for a family known to have cystic fibrosis is shown below. a half shaded circle is shown connected by a straight line to an unshaded square. underneath the half shaded circle are the letters aa which represent the genotype for the first parent. underneath the second parent the letters, aa, appear representing the genotype for the other parent. below this is another row of circles and squares. this 2nd row represents the offspring. starting from the left a half shaded circle is shown connected to an unshaded square, followed by an unshaded square, and then an unshaded circle. each of the circles and squares, offspring, are connected by a horizontal straight line that connects to the parent group above. the genotype is written under each of the offspring in order from left to right; aa, aa, aa, aa. what percentage of the offspring have observable disease? 0% 25% 50% 75% question 8(multiple choice worth 3 points) (06.03 lc) which of the following is an example of a homozygous recessive allele? rr rr rr rw question 9(multiple choice worth 3 points) (06.03 mc) the pod color in pea plant can be yellow or green. a pea plant with yellow pods (yy) is crossbred with another pea plant with green pods (yy). what is the probability of offspring with green pods? 0% 25% 50% 75% question 10(multiple choice worth 3 points) (06.02 mc) a pea plant has white flowers. it has a genotype pp. which of the following about the gene of flower color in the pea plant is true? the allele for white flowers is dominant in the pea plant. the allele for white flowers is recessive in the pea plant. the plant received both dominant alleles from both the parents. the plant received a dominant and a recessive allele from each parent. the characteristics of certain cell divisions are described in the following table. cell division characteristics characteristic description 1 forms diploid cells 2 creates sex cells, or gametes which type(s) of cell division do the two characteristics represent? both characteristics represent meiosis. both characteristics represent mitosis. characteristic 1 represents meiosis and characteristic 2 represents mitosis. characteristic 1 represents mitosis and characteristic 2 represents meiosis.

Facilitates growth and change in neurons and the migration of neurons to different sites in the brain