g You want to study a eukaryotic protein in a cell-free reaction system (just protein in buffer), so you first need to purify your protein. You clone the gene and its promoter region, then incorporate it into a plasmid. After transforming the plasmid into bacterial cells, you note that general protein expression has initiated but your protein is not observed after homogenizing the cells and analyzing the lysate. Why might this be true and how would you resolve it

What kind of bond is created by the attraction between atomic particles of opposite charge? ionic bond hydrogen bond covalent bond nuclear bond

You have just sequenced a new protein found in mice and observe that sulfur-containing cysteine residues occur at regular intervals. what is the significance of this finding? it will be important to include cysteine in the diet of the mice. cysteine residues are required for the formation of α helices and β pleated sheets. cysteine residues are involved in disulfide bridges that form tertiary structure. cysteine causes bends, or angles, to occur in the tertiary structure of proteins.

What's wrong with this ecological pyramid? (multiple choice)1. secondary consumers should be at the bottom of the pyramid2. the sun has an arrow leading to decomposers 3. primary consumers should come after the sun4. energy retained should increase from the bottom to the top5. the sun should be at the bottom of the pyramid