How many base pairs long are the E. coli O157:H7 DNA fragments that were separated out on the gel? (there are more than one) How many base pairs long was the original E. coli O157:H7 gene before it was cut using a restriction enzyme?

Consider a random mutation in a restriction site for one of the patient's samples that you ran. Would the banding pattern match the positive control sample? Explain how you determined your answer?

Lane 1 is a molecular base pair ladder, Lane 2 is a positive control for E. coli O157:H7, Lane 3 is patient 1, lane 4 is patient 2, lane 5 is patient 3 and lane 6 is patient 4.