Biology, 27.10.2021 01:00 babytony1210
You want to insert an ORF into pT5 plasmid. The DNA fragment is an open reading frame with ATG on the 5’end. pT5 plasmid contains a bacterial promoter that spans from 0.5 to 0.8 kb and goes clockwise, an EcoRI site at 0/6 kb, a HindIII site at 1 kb, a BamHI site at 3 kb. Your ORF is a HindIII fragment (2 kb long) with an EcoRI site at 1 kb and a BamHI site at 1.5 kb.
Required:
a. Draw the recombinant plasmid to show the right orientation of the ORF in the plasmid.
b. Which enzyme can you use to find out the orientation of the ORF after ligation?
Answers: 2
Biology, 22.06.2019 04:30
Study this image which statement best describes the rock shown check all that apply
Answers: 3
Biology, 22.06.2019 09:00
Which of these is an example of land degradation? a. containers designed to store pollutants leak. b. fertilizers provide too many nutrients to crops. c. garbage is buried so the land can be reclaimed later. d. a drought kills all the plants in an area, leaving bare land
Answers: 3
Biology, 22.06.2019 15:00
Located at the epidermal-dermal junction of the skin. select all true statements about the transformation of melanocytes into abnormal cell
Answers: 3
Biology, 22.06.2019 15:30
Bio molecules are essential to all living things. which biomolecule is “d” from the table
Answers: 3
You want to insert an ORF into pT5 plasmid. The DNA fragment is an open reading frame with ATG on th...
English, 01.03.2021 22:00
Mathematics, 01.03.2021 22:00
Mathematics, 01.03.2021 22:00
Mathematics, 01.03.2021 22:00
Mathematics, 01.03.2021 22:00
Mathematics, 01.03.2021 22:00
Mathematics, 01.03.2021 22:00
Biology, 01.03.2021 22:00
Mathematics, 01.03.2021 22:00
Mathematics, 01.03.2021 22:00
Mathematics, 01.03.2021 22:00
Mathematics, 01.03.2021 22:00
Social Studies, 01.03.2021 22:00