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According to the law of conservation of energy, energy can neither be created nor destroyed. if this is true, why is there less energy in the top of the energy pyramid than there is in the bottom of the energy pyramid?

You have been asked to lead a demonstration for the undergraduate microbiology lab course about the uses of negative staining when studying bacteria. a "negative" stain does not stain the bacterial cell itself but stains the space between cells. under magnification, the acidic (negativelycharged) nature of the stain will be repelled by the negatively charged bacterial cell wall and willleave the cell colorless in a stained background. negative stains are used primarily to reveal the presence of negatively charged bacterial capsules; therefore, they are also called capsule stains. encapsulated cells appear to have a halo surrounding them. the negative stain procedure does not require heat fixation, which limits any chances of alteration in bacterial cell shape and size. the bacterial suspension is added to a drop of stain, such as nigrosin or eosin, and drawn across the glass slide using a coverslip.nigrosin staining-not safranin staining-of klebsiella pneumoniae will allow for the visualization of the cell shape and the determination of the presence of a capsule.true/false

Glucose is a form of sugar found in the blood cells use glucose as a source of energy, but too much or too little can cause serious health issues so, the body uses the hormone insulin to regulate glucose n the blood insulin maintain glucose levels in the blood if blood glucose lovels got very high, what would you expect to see happen to insulin levels?