Which of the following is a step in gene cloning? a. The use of restriction enzymes to cut DNA at specific location
b. Selection of bacteria with the recombinant DNA
c. Transformation of bacteria with the recombinant DNA
d. All of the above

d. all of the above

answer: - few nutrients

high pressure

low temperatures

explanation: 1. few nutrients:   open-ocean zone is located way far from the land, which is the main source of the essential nutrients.2. high pressure: pressure increases by 1 atmosphere for every 10 meters increase in depth.3. ample sunlight: a large fraction of the sunlight is reflected back to the atmosphere from the sea surface. 4. varying salinity: below the thermocline, the water is isolated from the atmosphere so the salinity remains stable over the year.5. low temperatures:   the temperature of open-ocean zone ranges from a low of -2°c to an average of 17°c