How are biotic and abiotic factors related? How do these factors differ?

You have been asked to lead a demonstration for the undergraduate microbiology lab course about the uses of negative staining when studying bacteria. a "negative" stain does not stain the bacterial cell itself but stains the space between cells. under magnification, the acidic (negativelycharged) nature of the stain will be repelled by the negatively charged bacterial cell wall and willleave the cell colorless in a stained background. negative stains are used primarily to reveal the presence of negatively charged bacterial capsules; therefore, they are also called capsule stains. encapsulated cells appear to have a halo surrounding them. the negative stain procedure does not require heat fixation, which limits any chances of alteration in bacterial cell shape and size. the bacterial suspension is added to a drop of stain, such as nigrosin or eosin, and drawn across the glass slide using a coverslip.nigrosin staining-not safranin staining-of klebsiella pneumoniae will allow for the visualization of the cell shape and the determination of the presence of a capsule.true/false

Which of the following can be assumed about the layers in areas 2 and 4

Two nursing assistants are in line to wash their hands at a hospital after leaving a patient in an isolation room. the first na washes her hands and then uses her hands to turn off the faucet before drying them with a paper towel. the second na turns on the water with her hands and then proceeds to wash and dry her hands with a paper towel. which statement best describes errors demonstrated by the nursing assistants? the first na did not ensure a clean hand-washing environment, and the second na did not properly turn off the water the first na did not properly turn off the water, and the second na did not ensure a clean hand-washing environment. the first na did not properly turn off the water, and the second na did not properly use paper towels. the first na did not properly use paper towels, and the second na did not ensure a clean hand-washing environment.