Biology, 10.09.2021 23:20 blahhhhhhh10
Because you suspect that your new bacterium is an important member of Antarctic biofilms, you are interested in how they regulate synthesis of extracellular polysaccharides (EPS). You understand the key role that EPS plays in biofilm formation, and that it is normally produced in response to quorum sensing. In a short period of time, you have bioengineered a strain of this bacterium in which the promoter for its EPS gene is fused to green fluorescent protein (gfp). Now you want to test whether the gene is affected by extracts containing quorum-sensing signals. Which of the following approaches is best suited for your experiment?
a. Flow cytometry
b. optical density measurements
c. Microscopy using a Petroff-Hausser chamber
d. Measurements of total dry weight
e. tracking total protein levels.
Answers: 3
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