Experiment: Antibiotic sensitivity There is well understood documentation of the effect of Tetracycline on the microorganism E. col. Scientist have synthesised a new antibiotic and have devised an experiment to see if E. coli is as sensitive to the new antiiotic as it is to the Tetracycline. They soak a sterile disc in tetracycline, another in the new antibiotic, and a third in sterile Dl water. On the bottom of the plate, using a sharple, they divide the plate into three sections. They flipped the plate over and then cover the surface of a nutrient agar (petri dish) with a solution containing E. Coli, In one section of the plate they place the disc soaked in Tetracycline, in the adjacent section they place the disc soaked in new antibiotic, and on the third section they place the disc soaked in Dl water. They oincubate the plates overnight and observe the growth the following day. Effective antibiotics inhibit bacterial growth around the disc. This area is known as the zone of inhibition. The diameter of the zones of inhibition will be measured for each disc and compared. Identify the parts of the experiment.
a. Disc soaked in Tetracycline
b. Diameter of the zone of inhibition
c. Disc soaked in the new antibiotic
d. The discs soaked in their respective solutions
e. Disc soaked in Dl water
1. Independent variable
2. Dependent variable
3. Positive control
4. Negative control
5. Experimental