Describe the role of enzymes in seeds germination​

Enzymes such as amylase ,protease and lipase are liable for solubilizing spare food materials in form of starch, protein and lipids correspondingly

in seed and deliver energy and other fundamentals food materials to germinating embryo.

The seed proteins are catalyzed by protease enzyme and break it into amino acid and peptides that a e transferred to growing embryo.

The amino acids obtained from metabolism of the proteins are further used in biosynthesis of enzymes,hormones,proteins, pyrimidines and Purine bases.

The starch is catalyzed by enzyme amylase that provides the food materials for growth and development of embryo . Similarly lipase are enzymes that are responsible for metabolism of the triacylglycerols into glycerols and fatty acids.This is also the source of energy for growing embryo

this protocol provides a detailed procedure for co-immunoprecipitation (co-ip) to identify protein–protein interactions.