What are the symptoms of PEM {Protein–energy malnutrition}
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Explanation:

Poor weight gain.

Slowing of linear growth.

Behavioral changes - Irritability, apathy, decreased social responsiveness, anxiety, and attention deficits.

this protocol provides a detailed procedure for total protein extraction for western blot analysis from achieved samples following trizol isolation of rna.

1. loamy sand is about 80% sand with 10% clay and defined mostly by sand particles with some clay to provide structure and fertility. the sand particles are easily visible and the soil holds structure but breaks apart easily.

2. scott sand can improve his soil quality by incorporating more silt and organic matter into the soil. peat moss and organic matter can also be incorporated.