B-DNA in a bacterial cell (E. coli) has the following characteristics: Wr = -7, Tw = +44.
What would be the numerical values (include + or - signs in your answers) for Wr, Tw and Lk for the DNA (i. e., for the most stable structure) in the cell after interacting with each of the following enzymes listed below (assume each enzyme carries out its function only one time on the given DNA molecule)? Note: assume B-DNA has 10 bp per helical turn.
E. coli Top I Wr = Tw = Lk =
E. coli DNA gyrase Wr = Tw = Lk =
E. coli Top IV Wr = Tw = Lk =