Students have been using a cell-free system that used room-temperature stable protein of interest. To stop the reaction, they use heat to the proteins to high temp then add negatively charged detergents. This allows the students to separate the enzymes, the protein targets, and their fragments on an electrophoresis gel. What happens to enzymes and other proteins when they are heated up and soaked in detergent? How would the larger enzymes separate from the protein fragments on gel? How would a protein that is cut look before and after the experiment? Describe the mechanism for gel electrophoresis.