You are trying to program E. coli bacteria to generate N-acylethanolamide, lipids that have been found to help suppress appetite. In order to do this, you need to transfer the gene for the enzyme N-acyltransferase from the plant Arabidopsis Thaliana into the E. coli bacteria. The transgene, which codes for , will also need to have a
for RNA polymerase and a that would fluoresce to let you know the insertion was successful. You would also need a , a small circular piece of bacterial DNA.
Then you would hunt for a that would cut the plasmid once and also cut the transgene out from its host.

FILL IN THE

You have been asked to lead a demonstration for the undergraduate microbiology lab course about the uses of negative staining when studying bacteria. a "negative" stain does not stain the bacterial cell itself but stains the space between cells. under magnification, the acidic (negativelycharged) nature of the stain will be repelled by the negatively charged bacterial cell wall and willleave the cell colorless in a stained background. negative stains are used primarily to reveal the presence of negatively charged bacterial capsules; therefore, they are also called capsule stains. encapsulated cells appear to have a halo surrounding them. the negative stain procedure does not require heat fixation, which limits any chances of alteration in bacterial cell shape and size. the bacterial suspension is added to a drop of stain, such as nigrosin or eosin, and drawn across the glass slide using a coverslip.nigrosin staining-not safranin staining-of klebsiella pneumoniae will allow for the visualization of the cell shape and the determination of the presence of a capsule.true/false

Agroup of students is studying convection currents. they fill two identical balloons with the same amount of helium. one balloon is placed in a freezer and the other in an area with warm air. after 10 minutes, the balloons are released from a height of 1 meter. which of the following do the students most likely observe? question 8 options: the cold balloon expands and rises. the warm balloon shrinks and sinks. the balloons rise at the same rate. both balloons are the same size. the ballons both rise. the cold ballon is larger than the warm balloon. the warm balloon expands and rises. the cold balloon shrinks and sinks.

What mistake did john needham make that caused him to conclude that spontaneous generation for microorganisms occurred? a. he re-contaminated his boiled broth solutions. b. he destroyed the vital force in the solutions. c. he did not boil his broth solutions, only warmed them. d. he failed to seal his flasks of boiled broth. e. he allowed his assistant to conduct the experiment which he did not monitor closely.