How many amino acids ended up being incorrect, or different from the original amino acid sequence, as a result of the substitution mutation?

A substitution mutation can be defined as a mutation in which a single nucleotide is replaced by the other. As a single nucleotide changes in this process which does not create an effect on the final protein so it is a least dangerous type of DNA mutation.

Explanation:

There are three types of substitution mutation:

1. Silent mutation: In this the replaced single base pair gets replaced by the mutated RNA sequence producing the same amino acid. If the nucleotide composition gets changed it will produce the same amino acid.

2. Missense mutation: The nucleotide composition gets changed as a result of addition of different amino acid after mutation in the protein.

3. A nonsense mutation involves the change in the stop codon. This results in non-functioning of the protein.

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