In the process of bacterial transformation, what is used to cut open a plasmid in order to insert the gene of interest? O A a restriction enzyme
B. helicase
c. a vector
D. polymerase

when you breathe in, or inhale, your diaphragm contracts (tightens) and moves downward. this increases the space in your chest cavity, into which your lungs expand. the intercostal muscles between your ribs also enlarge the chest cavity. they contract to pull your rib cage both upward and outward when you inhale.

maybe because its impossible for balloons to lift a whole entire house.