Evidence supporting E. coli DNA polymerase III having the major role in nucleotide incorporation during replication includes: Evidence supporting E. coli DNA polymerase III having the major role in nucleotide incorporation during replication includes: A point mutation can inactivate DNA pol III and inhibit replication. DNA polymerase III has a high Vmax. There are ten or fewer molecules of enzyme per cell. Replication is blocked at high temperatures in temperature sensitive mutants with a thermolabile form of DNA polymerase III. All of the above.