The active ingredients in many pesticides are chemical compounds that kill organisms such as insects, molds, and weeds. Proponents claim that the use of pesticides improves crop yields and thus protects land and soil by reducing the conversion of forests and wetlands to cropland. Opponents of pesticide use claim that pesticides degrade water and soil quality and that other modern agricultural techniques and practices are responsible for the improved crop yields in recent years. Design a laboratory experiment to determine whether or not a new pesticide (product X) is toxic to minnows, a type of small fish.

Penicillin is hydrolyzed and thereby rendered inactive by penicillinase (also known as β-lactamase), an enzyme present in some resistant bacteria. the mass of this enzyme in staphylococcus aureus is 29.6 kd. the amount of penicillin hydrolyzed in 1 minute in a 10-ml solution containing 10-9 g of purified penicillinase was measured as a function of the concentration of penicillin. assume that the concentration of penicillin does not change appreciably during the assay. penicillin(μm) amount hydrolyzed(nmol) 1 0.11 3 0.25 5 0.34 10 0.45 30 0.58 50 0.61 (a) plot v0 versus [s] and 1/v0 versus 1/[s] for these data. does penicillinase appear to obey michaelis-menten kinetics? if so, what is the value of km? (b) what is the value of vmax? (c) what is the turnover number of penicillinase under these experimental conditions? assume one active site per enzyme molecule.