Biology, 03.04.2021 04:50 emblemhacks
Blue-white screening is a technique for the identification of___. It relies on the ability ofto cleave lactose. Blue-white tests take advantage of a molecule calledwhich is similar to lactose in that it is cleaved by B-galactosidase. When cleaved, the(same as previous) turns . If uncleaved, meaning a non- function B-gal gene, the X-gal remains. Therefore, abacterial colony means the B-galactosidase gene is not-functional, and therefore therea recombinant gene inserted into the vector. Youβve inserted a gene for pesticide resistance into your vector. To see if it was successful, you decide to conduct a blue-white screening test. But, uh-oh you are out of X-gal! You do notice some green Slime-gal sitting on the shelf and recall that this molecule works similarly to X-gal, but instead of turning blue when cleaved, it turns green when cleaved. You carry out the test, and find that all of your colonies are green! Was your insertion of the pesticide gene successful?
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Biology, 21.06.2019 17:10
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Blue-white screening is a technique for the identification of___. It relies on the ability ofto clea...
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