You are interested in whether the protein you are studying for your dissertation, ZyxR, is capable of binding to and regulating expression of the ZyxABC operon. ZyxR is a 24kDa protein with a slightly negative overall charge and no known inherent tags. ZyxR is known to bind to the zyxY gene to regulate expression. yopA (100bp) is not regulated by ZyxR. The first step in this process is to determine whether ZyxR is capable of binding to the regulatory regions surrounding the zyxABC operon. Give one potential strategy that you could use to purify ZyxR. Your strategy should be a multistep process beginning with cell lysate (containing millions of proteins) that you will take to purify the protein, should address potential shortcomings of each step, and should attempt to rectify those shortcomings. If your strategy requires any special conditions or changes to ZyxR, you should include those in the details. Drawings of your strategy/steps are highly encouraged!

Explanation:

One potential methodology which could be utilized to cleanse ZxyR is Affinity chromatography. Affinity chromatography is utilized for the most part relevant methods for cleansing proteins. This strategy exploits the high harmony of numerous proteins for explicit synthetic gatherings. For instance, the plant protein concanavalin A can be filtered and purified by going a rough concentrate crude extract through a segment of beads encapsulating covalently joined glucose deposits.

americans began to notice growing differences between the northern and southern sections of the country. there were more people living in cities in the north, which made it more urban. more people lived on farms in the south, which made it more rural. while many people were farmers in the northern states, there were now other ways to make a living, such as working in mechanical cotton gins factories manufacturing cloth, tools and other goods. people in the north supported high tariffs on goods imported from europe because the extra cost encouraged people to “buy american” instead. in the southern states, planters did not like tariffs because they sold most of their production to europe and bought their tools from there as well. southerners believed the protective tariff was something that only northern factory owners keep their profits high. so the southern politicians came up with an idea. they would opt out. they would simply ignore the protective tariff. it was ‘null and void’ in south carolina. the state would not collect the taxes on goods imported into south carolina, no matter what the president said they had to do. this decision led to the nullification crisis.

the correct answer is 4) heat and pressure

metamorphic rocks arise from transformation of existing rocks types, in a process called metamorphism. during metamorphism the original rock(prolith) change into metamorphic rock by heating (150-200c) and pressure of more than 1000 bar. the original rock can be sedimentary, igneous, or existing metamorphic rock.