Aconfined aquifer is
a) found in the unsaturated zone
b) found between two impermeable layers
c) the top layer of the saturated zone
d) separated from the main groundwater

Aquifers are underground layers of rocks which contain water and serve as important sources of freshwater. They are located below the water table and in the saturated zone. If an aquifer becomes trapped between two impermeable layer (for example, one below and one above that overlies the aquifer), the pressure act on the aquifer and it can become a confined aquifer.

A confined aquifer is found between two impermeable layers.

this protocol provides a detailed procedure for agarose-based ip to purify and enrich the protein of interest.