One method for genome editing, called CRISPR, is a process first discovered in immune defense in prokaryotes. This process involves an endonuclease enzyme, , that is capable of identifying sequences of DNA. The enzyme uses a(n) that complementary base pairs with a(n) . In prokaryotes, this method protects from invading viruses. After the DNA sequence is identified, produces a break in both strands of the viral DNA. A strand of DNA adjacent to the target sequence, called , protects the host DNA from damage. This process can be replicated to target specific DNA for . The break in the DNA can be used to a gene to study its role in a cell, or to at specific genomic locations.

autosomal

explanation:

is a term used in genetic genealogy to describe dna which is inherited from the autosomal chromosomes

this protocol provides a detailed procedure for nucleoprotein extraction from cultured cells and tissues in an efficient manner.

within 30 of the equator hope this

cells use diffusion to get substances in and out of themselves. that diffusion will occur across the cell membrane or the outer covering of the cell. because we are talking about the surface of the cell, surface area and volume are  important factors here. as a cell increases in size, its surface area and volume increase as well.  we call that the surface area to volume ratio (sa: vol).  but the catch is that  volume increases at a greater rate than surface  area. in other words, as a cell gets bigger, the sa: vol gets smaller. so a really small cell like bacteria is mostly cell membrane (surface) with very little contents (volume). a really big cell like an egg, is mostly insides with a super thin membrane. so what ends up happening is that the cell cannot efficiently diffuse all of the stuff it needs in/out to survive. therefore cell size is limited to about 100mm. so how do we make organisms bigger than 100mm? cells divide! by dividing and becoming multicellular, organisms are able to grow!   check out the diagrams i attached to see the effect of sa: vol. i hope this ! good luck!