In order to initiate transcription in eukaryotes, . View Available Hint(s) In order to initiate transcription in eukaryotes, . the RNA polymerase simply binds to the promoter and begins to transcribe the gene helicase unwinds the DNA double helix to make room for RNA polymerase to extend the primase enzyme lays down a primer, and then the polymerase enzyme begins to copy the sequence by extending the primer transcription factors bind to the promoter, and RNA polymerase is then recruited to begin transcribing the gene