Methods that can be used to determine the variety of organisms in water suggest two limitations of this method

analyse the affect of sample size in the result of this method
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Parthenogenesis is a type of reproduction that does not require a mate. it’s rarely seen in birds and higher vertebrates. parthenogenesis involves the formation of a zygote. but this zygote is formed without fertilization. in parthenogenesis, in the absence of a male gamete, the ovum develops directly in the zygote.