You have a circular plasmid containing 9 kb of DNA, and you wish to map its EcoRI and BamHI sites. When you digest the plasmid with EcoRI and run the DNA on a gel, you observe a single band at 9 kb. You get the same result when you digest the DNA with BamHI. When you digest with a mixture of both enzymes, you observe two bands, one 6 kb and the other 3 kb in size. Explain these results. Draw a map of the restriction sites.

A nick is created between G and A in GAATTC when using Eco R1 site while a nick is generated between G and G in GGATCC when Bam H1 is used. The difference in the nicks created is the main reason you observe two bands when you digest the circular plasmid with a mixture of both

Explanation:

Circular plasmid containing : 9 kb of DNA

Eco R1 and Bam H1 are known as restriction sites in DNA ( i.e. special sequences found in a DNA ) hence they can  be specifically cleaved by the restriction enzymes enabling the insertion of a gene. that is why you will get a same result when you digest the Plasmid with either Eco R1 or Bam H1

but A nick is created between G and A in GAATTC when using Eco R1 site while a nick is generated between G and G in GGATCC when Bam H1 is used. The difference in the nicks created is the main reason you observe two bands when you digest the circular plasmid with a mixture of both

answer: after carbon dioxide enters the leaf through stomata it moves into the mesophyll cells where photosynthesis occurs and glucose is constructed. respiration occurs when glucose (sugar produced during photosynthesis) combines with oxygen to produce useable cellular energy.

explanation: