Biotechnology has allowed us to genetically modify corn with a gene that acts as a pesticide. what could be a possible environmental impact of this development?

Since a gene that acts as a pesticide is inserted into a corn, that corn is now safe from insects. This will happen because the corn will probably synthesise protein according to that inserted DNA sequence and that protein will serve as a repellent to the insects. However, the Red Queen's hypothesis in evolution explains that organisms must constantly adapt and evolve in order to survive. This refers to insects. If they could not eat genetically modified corn, they would extinct. So, they must adapt or evolve in order to survive. So, it is expected that insects that are resistant to this specific pesticide would develop through the time.

The answer is joseph is observing the colour of the reaction mixture to see whether proteins are present in the given solution.

Mitochondria produce atp, a high energy compound. atp is used for all sorts of enzyme-mediated reactions, such as making proteins/nucleotides/etc, certain compounds cross the cell membrane, muscles move, etc.  without this source of energy, a cell will die. it will not be able to repair itself, defend against pathogens, etc.