Plz help i will give brainlist

Could someone me with number one you

What causes darkening of the skin as melanin production increases

Ayoung boy has been found and police are trying to locate his family they take a dna sample from him and begin collec dna samples from families who have missing children if police use dna samples only from the fathers, which type of dn technology can they use to identify the boy's parent? y-chromosome analysis omtdna (mitochondrial dna) analysis vntrs (variable tandem repeats) o pcr (polymerase chain reaction) analysis