You are studying expression of your favorite gene, YFG1. You have cloned the entire gene including its promoter to a plasmid. In addition to the wild type, you have also made mutations to two different parts of the gene that you suspect are the promoter. Let's call these mutants Mut1 and Mut2. After transforming these 3 plasmids into separate cultures of cells, describe an assay you could use to measure the amount of RNA produced, and how you would know if Mut1 or Mut2 have mutations in the promoter. Show what ideal results would look like. I want details and specifics - this is not a question you can answer with 2-3 sentences. (recommend minimum 300 words)

Select the correct answer. in the image, if the washcloth represents consumed food, which organ of the digestive system correctly represents the action performed by the hands? hands squeezing water from a washcloth a. esophagus b. large intestine c. liver d. mouth

Read the following scenario to answer the following question. over the past 60 years, many amphibian species have experienced significant population declines, and some species have become extinct. scientists suspected that local human activities such as the destruction of wetlands, regional pollution, and deforestation were the main reasons for these losses. however, research over the past 20 years reveals significant amphibian population declines in protected areas of the world, such as nature preserves and parks. these global declines suggest widespread problems including increased ultraviolet radiation, acid rain, and disease. in switzerland, for example, 14 of the 20 native amphibian species are threatened with extinction. when most populations of a wide-ranging amphibian species are lost and the few remaining populations are widely separated, we expect to see that a. the founder effect becomes increasingly important b. microevolution no longer occurs c. gene flow between populations is reduced d. artificial selection becomes a greater factor in microevolution

What are some characteristics of a free enterprise economy?