You plan to clone a guide RNA sequence into the pML104 plasmid because you want to engineer the construct in bacteria and later express it in yeast. Unfortunately, your lab does not have any available pML104. Your friend in the lab next door lets you borrow a related plasmid pML107, and you quickly start your experiment. Will it work? Briefly explain.

Yes, the experiment will work.

Explanation:

The reason behind this is that plasmids are well known to be DNA vector carriers. These plasmids have the ability to bring about changes in DNA fragments into various microorganisms and reveal them. The two plasmids compose of the same genomic marker such as the antibiotic resistance gene found in URA3. However, plasmids are most commonly found in bacteria, but also in eukaryotic cells. Oftentimes, the gene composition in the plasmids enhances the bacteria with a vantage edge such as antibiotic resistance.

Hence, it is obvious that in both, the molecular regulation in terms of expression of genes is alike in properties. Similarly, the numbers of copies and molecular weight are also the same.

We can thereby conclude that, provided that there is a proper restriction site, another host that is up to standard for plasmid can be replaced for each other.

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