How is tone in poetry defined?

The poet's attitude toward the poem's speaker, reader, and subject matter, as interpreted by the reader. Often described as a “mood” that pervades the experience of reading the poem, it is created by the poem's vocabulary, metrical regularity or irregularity, syntax, use of figurative language, and rhyme.

Explanation:

this protocol provides a detailed procedure for agarose-based ip to purify and enrich the protein of interest.