What determines retention factor (Rf) ?

Rf valuues and reprroooducibility can be affected by a number of different factors such as layer thickness, moisture on the TLC plate, vessel saturation, temmperature depth of mobile phase, nature of the TLC plate sample size, and solvent parameters. These effects normally cause an increase in Rf values

Explanation:

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when atoms of different types of elements join together, they make molecules called compounds. water consists of compound molecules made up of 2 hydrogen atoms and 1 oxygen atom. this is why it's called h2o. water will always have 2 times the number of hydrogen atoms as oxygen atoms.