Ome reptiles such as crocodilians and some turtles are known to display temperature-dependent sex determination (TSD), where the ambient temperature of the developing eggs at a crucial point of development determines the individual's sex/gender. An international joint research team has determined that the thermosensor protein TRPV4 is associated with temperature-dependent sex determination (TSD) in the American alligator.

Using this information, which of these statements is a valid conclusion?

Translation cannot occur at certain temperatures.
A chromosomal mutation has occurred producing only male gametes.
Environmental conditions can affect gene expression/protein synthesis.
A point mutation occurs only at certain temperatures.