What occurs at step 3 in the diagram? O The restriction enzymes cut the isolated DNA into fragments
O The gene of interest is inserted into an expression cassette.
O The recombinant DNA is inserted into the host cell nucleus via a vector.
O The expression cassette is inserted into a plasmid for cloning

Which of the following observation darwin shape his concept of descent with modification? a) species diversity declines farther from the equator. b) fewer species live on islands than on the nearest continents. c) birds live on islands located farther from the mainland than the bird's maximum nonstop flight distance. d) south american temperate plants are more similar to the tropical plants of south america than to the temperate plants of europe. e) earthquakes reshape life by causing mass extinctions.