The diagram below represents the distribution of some molecules inside and outside of an artificial cell over a period of time . Which statement is best supported by the diagram?

1. Oxygen molecules entered the cell over time by active transport.
2. Water molecules are too large to enter or leave the cell, so they remained where they were at the start of the investigation.
3. Protein molecule are kept inside of the cell because the cell needs them.
4. The protein molecules are too large to diffuse out the cell.