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Salivary amylase is a protein secreted by the epithelial cells of the salivary glands to aid in carbohydrate digestion. Order the steps below for the generation of amylase in their correct order.
The amylase polypeptide folds into a protein inside the ER lumen and is packaged in a vesicle destined for the Golgi
A release factor binds a stop codon on the amylase mRNA
Vesicles containing amylase leave the Golgi and fuse with plasma membrane to secrete protein from the cell
The growing polypeptide chain continues to insert itself inside the lumen of the ER
Ribosome is fully assembled and translation elongation proceeds
The tRNA charged with Met amino acid recognizes the AUG on the amylase mRNA bound to small ribosomal subunit
RNA polymerase binds to the amylase gene promoter
A mature mRNA is exported out of the nucleus
RNA elongation proceeds until RNA polymerase falls off the DNA
A poly A tail is added to the 3' of pre-mRNA
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