A researcher studying llama genetics would like to clone her gene of interest into the pUC19 cloning vector using the blue-white screening approach we discussed in class. Below are the vector and llama DNA fragment to be cloned into the vector. The restriction enzyme sites indicated on both DNA molecules. Which restriction enzyme should she use in order to get the largest possible DNA fragment cloned into the vector

How do the nuclei of covalently bonded atoms keep the bond together

State officials are considering constructing a road through a forested wilderness area. this action will likely affect the forest ecosystem in various ways. part a: predict how the construction of a road could negatively affect plants and animals in that ecosystem. (3 points) part b: describe one way that the construction of a road could have a positive impact of the forest ecosystem. (1 point)

What is the significance of the protein-lined pits? a. protein attracts other proteins needed for atp synthesis within the cell. b. protein-lined pits are able to transport one molecule at a time down the concentration gradient within the cell. c. the polarity of proteins allows other polar molecules to attach and be transported in the cell by transport channels. d. receptors within the pits allow ligands to fuse and be transported into the cell by endocytosis.