3.We anticipate our recombinant DHFR protein to be functional for use in future research applications. However, if you had been able to test functionality and found that your recombinant GST-DHFR-His protein was not functional, what would you propose is one potential issue you would investigate to troubleshoot further. In other words, what is one thing that could have gone wrong to result in non- functional protein from the method used and results obtained this semester

In the given case, the protein would have required to go through the process of post-translational modification, however, the mentioned protein would have been cultured in the prokaryotic system, the process of post-translational modification would have failed to take place. This step would be essential for the functionality of the protein. Thus, there is a need to culture it within the eukaryotic system.

The covalent and generally the enzymatic modification of proteins post its biosynthesis is termed as post-translational modification.

white blood cells or wbc s are mainly 2 types-

1.agranulocytes

2.granulocytes

the monocytes or macrophages of agranulocytes , dendritic cells which are accessory leucocytes, and b cells which is one type of lymphocytes   identify the antigens present on the pathogen's surface. these three cells are called antigen presenting cells(apc) which are phagocytes and destroy the antigens by secreting antitoxins. besides this leukocyte is always plays vital role in the immunity of the body.

1. dermis

2. chemical

3. urine

4. viruses

5. antibodies